Anxiolytic-like effects and toxicological studies of Brickellia cavanillesii (Cass.) A. Gray in experimental mice models

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• 作者：Gabriela &Aacute ; vila-Villarreal^a ; ¹ ; Marí ; a Eva Gonz&aacute ; lez-Trujano^b ; Azucena Ibeth Carballo-Villalobos^b ; Berenice Aguilar-Guadarrama^c ; Sara Garcí ; a-Jimé ; nez^a ; Diana Elizabeth Giles-Rivas^a ; Patricia Castillo-Españ ; a^d ; Rafael Villalobos-Molina^e ; Samuel Estrada-Soto^a ; ^{enoch@uaem.mx" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Anxiolytic-like effect ; Acacetin ; Brickellia cavanillesii ; Luteolin ; Nicotiflorin ; Toxicological studies
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 November 2016
• 年：2016
• 卷：192
• 期：Complete
• 页码：90-98
• 全文大小：2913 K

文摘

Brickellia cavanillesii (Asteraceae) (Cass.) A. Gray is one of the popular plants consumed in Central America and Mexico for the treatment of several diseases such as hypertension, diabetes and anxiety, among others.

Aim of the study

To determine the anxiolytic-like effect of B. Cavanillesii and the safety of its use through toxicological studies.

Material and methods

Anxiolytic-like effects of soluble-methanol extract of B. cavanillesii (MEBc) were evaluated in ambulatory activity (open-field test), hole-board test, cylinder of exploration, the elevated plus-maze and the potentiation of the sodium pentobarbital-induced hypnosis mice models. On the other hand, in vivo toxicological studies were conducted on acute and sub-acute mice models recommended by OECD. Active MEBc was subjected to phytochemical studies through conventional chromatographic techniques to isolate bioactive compounds.

Results

MEBc (100 mg/Kg) showed significant anxiolytic-like effect on animal model used (p<0.05). The phytochemical analysis of MEBc allowed the isolation of two major compounds nicotiflorin and acacetin, among others. Both compounds were found to be partially responsible for the anxiolytic-like effects. Moreover, a median lethal dose (LD₅₀) higher than 2000 mg/Kg was determined in mice and sub-acute oral administration of MEBc (100 mg/Kg) did not alter body weight, clinical chemistry parameters (ALT and AST) and it did not induce any toxic nor alteration in the liver, kidney and heart functions.

Conclusions

In current investigation, we have shown that MEBc has a wide range of pharmacology–toxicology patterns. The results support further investigation of MEBc as a potential anxiolytic phytomedicinal agent.