Full-Length Anion Exchanger 1 Structure and Interactions with Ankyrin-1 Determined by Zero Length Crosslinking of Erythrocyte Membranes

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• 作者：Roland Rivera-Santiago¹ ; Sandra L. Harper¹ ; Sira Sriswasdi² ; Peter Hembach¹ ; David W. Speicher¹ ; ³ ; ^{speicher@wistar.org}
• 关键词：anion exchanger 1 ; erythrocyte membrane ; chemical crosslinking ; mass spectrometry ; structural mass spectrometry ; homology modeling ; ankyrin ; protein-protein interactions ; red cell membranes ; band 3
• 刊名：Structure
• 出版年：2017
• 出版时间：3 January 2017
• 年：2017
• 卷：25
• 期：1
• 页码：132-145
• 全文大小：4389 K
• 卷排序：25

文摘

Zero-length crosslinking of intact cell membranes was used to probe AE1 structure A full-length AE1 model was deduced using crosslinks and known partial structures Previously unresolved loops form extensive contact between the two major domains Protein-protein interaction sites are more extensive than reported previously