Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of human breast cancer cells MCF-7

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• 作者：Shanmugapriya^a ; Yeng Chen^b ; Jagat Rakesh Kanwar^c ; Sreenivasan Sasidharan^a ; ^{srisasidharan@yahoo.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Antiproliferative activity ; Human breast cancer ; CyQuant assays ; Giemsa staining
• 刊名：Asian Pacific Journal of Tropical Disease
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：6
• 期：4
• 页码：291-297
• 全文大小：966 K

文摘

To evaluate the antiproliferative activity of m>Calophyllum inophyllumm> (m>C. inophyllumm>) fruit extract against human breast cancer cells MCF-7.

Methods

The cytotoxic effect of m>C. inophyllumm> fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays for 24 h and the morphological investigation of treated MCF-7 cells was observed under optical microscope using Giemsa staining.

Results

The cytotoxic effect of m>C. inophyllumm> fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays simultaneously for 24 h after treatment, which demonstrated the inhibition of cell viability with the IC₅₀ values of 19.63 μg/mL and 27.54 μg/mL, respectively. The preliminary time-based morphological investigation of MCF-7 cells treated with the IC₅₀ value (23.59 μg/mL) of m>C. inophyllumm> fruit extract was observed under an optical microscopy via Giemsa staining, which exhibited prominent histological characteristics of apoptosis.

Conclusions

This study clearly proved that the proliferation of human breast cancer cell MCF-7 was inhibited by m>C. inophyllumm> fruit extract resulted from the induction of apoptosis in MCF-7 cells.