Murine transgenic iPS cell line for monitoring and selection of cardiomyocytes

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• 作者：Azra Fatima ; Guoxing Xu ; Filomain Nguemo ; Alexey Kuzmenkin¹ ; Karsten Burkert ; Jü ; rgen Hescheler ; Tomo &Scaron ; arić ; ^{tomo.saric@uni-koeln.de" class="auth_mail" title="E-mail the corresponding author}
• 刊名：Stem Cell Research
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：17
• 期：2
• 页码：266-272
• 全文大小：1616 K

文摘

We report here a transgenic murine induced pluripotent stem cell (iPSC) line expressing puromycin N-acetyltransferase (PAC) and enhanced green fluorescent protein (EGFP) under the control of α-myosin heavy chain promoter. This transgenic cell line reproducibly differentiates into EGFP-expressing cardiomyocytes (CMs) which can be generated at high purity with puromycin treatment and exhibit molecular and functional properties of immature heart muscle cells. This genetically modified iPSC line can be used for assessment of the utility of CMs for myocardial repair, pharmacological and toxicological applications and development of improved cardiac differentiation protocols.