Application of core–shell PEGylated CdS/Cd(OH)₂ quantum dots as biolabels of Trypanosoma cruzi

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• 作者：C.R. Chaves ; A. Fontes ; P.M.A. Farias ; B.S. Santos ; F.D. de Menezes ; R.C. Ferreira ; C.L. Cesar ; A. Galembeck ; R.C.B.Q. Figueiredo
• 关键词：Quantum dots ; Fluorescence ; Parasites ; Trypanosoma cruzi
• 刊名：Applied Surface Science
• 出版年：2008
• 出版时间：30 November 2008
• 年：2008
• 卷：255
• 期：3
• 页码：728-730
• 全文大小：342 K

文摘

Semiconductor quantum dots are a promising class of materials in the labeling of biological systems. In the present study we show the marking pattern of Trypanosoma cruzi (T. cruzi) live parasites using PEGylated CdS/Cd(OH)₂ fluorescent nanocrystals. The analysis obtained by confocal fluorescence microscopy and transmission electron microscopy indicates that only the endocytic paths of parasites were labeled. The parasites were alive after the incubation with the CdS/Cd(OH)₂–PEG suspension. Labeling the T. cruzi with quantum dots can help to better understand the endocytosis process and also the cellular differentiation.