An international multicenter study on HIV-1 drug resistance testing by 454 ultra-deep pyrosequencing
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- 作者：Birgitte B. Simena ; Michael S. Bravermana ; Isabella Abbateb ; Jeroen Aerssensc ; Yannick Bidetd ; Olivier Boucheze ; Christian Gabrielf ; Jacques Izopetg ; Harald H. Kesslerh ; Evelyn Stelzlh ; Francesca Di Giallonardoi ; Ralph Schlapbachj ; Aleksander Radonick ; Roger Paredesl ; Patricia Recordon-Pinsonm ; James Sakwan ; Elizabeth P. St. Johna ; Gudrun G. Schmitz-Agheguiano ; Karin J. Metzneri ; Karin.Metzner@usz.ch" class="auth_mail ; Martin P. Dä ; umerp ; on behalf of the 454 HIV Alphastudy Group
- 关键词：HIV-1 ; Drug resistance ; 454 ultra-deep pyrosequencing ; Multicenter study ; Next-generation sequencing
- 刊名：Journal of Virological Methods
- 出版年：August 2014
- 年：2014
- 卷：204
- 期：Complete
- 页码：31-37
- 全文大小：852 K
文摘
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First multicenter study on HIV-1 drug resistance testing by 454 ultra-deep pyrosequencing to evaluate reproducibility and concordance of this new protocol.
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All HIV-1 drug resistance mutations identified by population sequencing were also identified by 454 ultra-deep pyrosequencing.
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Minority HIV-1 drug resistance mutations were only detected by 454 ultra-deep pyrosequencing.
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454 ultra-deep pyrosequencing provided highly concordant results together with excellent cross-site correlation.