Benzo[a]pyrene degradation by Sphingomonas yanoikuyae JAR02

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• 作者：Jeremy A. Rentz ; Pedro J.J. Alvarez ; Jerald L. Schnoor
• 关键词：Salicylate ; Biostimulation ; Biodegradation ; Mineralization
• 刊名：Environmental Pollution
• 出版年：2008
• 出版时间：February 2008
• 年：2008
• 卷：151
• 期：3
• 页码：669-677
• 全文大小：329 K

文摘

Batch experiments were conducted to characterize the degradation of benzo[a]pyrene, a representative high molecular weight (HMW) polycyclic aromatic hydrocarbon (PAH), by Sphingomonas yanoikuyae JAR02. Concentrations up to the solubility limit (1.2 μg l⁻¹) of benzo[a]pyrene were completely removed from solution within 20 h when the bacterium was grown on salicylate. Additional experiments with [¹⁴C]7-benzo[a]pyrene demonstrated 3.8 % mineralization over 7 days when salicylate was present is solution, and one major radio-labeled metabolite was observed that accounted for 10 % of the initial radio-label. Further characterization of the radio-labeled metabolite using HPLC/MS and HPLC/MS/MS identified radio-labeled pyrene-8-hydroxy-7-carboxylic acid and unlabeled pyrene-7-hydroxy-8-carboxylic acid as novel ring-cleavage metabolites, and a benzo[a]pyrene degradation pathway was proposed. Results indicate that biostimulation of HMW PAH degradation by salicylate, a water-soluble, non-toxic substrate, has significant potential for in situ bioremediation.