Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models

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• 作者：Guoliang Sa^a ; Xuepeng Xiong^a ; ^b ; Jiangang Ren^a ; Yifang Zhao^a ; ^b ; ^{yifang@whu.edu.cn} ; Sangang He^a ; ^b ; ^{sangang@whu.edu.cn}
• 关键词：Oral submucous fibrosis ; Animal model ; TGF-β ; CCN2 ; Extracellular matrix
• 刊名：European Journal of Pharmaceutical Sciences
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：472-478
• 全文大小：2107 K
• 卷排序：96

文摘

Several animal models have been used in studies associated with oral submucous fibrosis (OSF); however, an appropriate model based on the histopathological characteristics of OSF is still needed. This study aimed to provide histological references for selecting a potential model. The expression intensities of collagen type I (Col I), type III (Col III), type IV (Col IV), fibronectin (FN), transforming growth factors β (TGF-β), and connective tissue growth factor (CCN2) in the oral mucosa of the human and six non-human animal species were measured by immunohistochemistry. There was little variation in the expression intensity of Col I while the expression of Col III, Col IV, and FN showed differences. The expression intensities of TGF-β in dog, rat, sheep, and pig oral mucosae, and those of CCN2 in dog, minipig, rat, and buffalo oral mucosae were equivalent to the expression intensities in human mucosa. The expression of fibrosis-related molecules in the dog oral mucosa optimally mimics the human condition, suggesting its suitability with regard to histopathology as an animal model for the study of OSF.