In vitro effect of zinc and cadmium on acetylcholinesterase and ectonucleotidase activities in zebrafish (Danio rerio) brain

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• 作者：Mario Roberto Senger ; Denis Broock Rosemberg ; Eduardo Pacheco Rico ; Marcelo de Bem Arizi ; Renato Dutra Dias ; Maurí ; cio Reis Bogo and Carla Denise Bonan
• 关键词：Zinc ; Cadmium ; Acetylcholinesterase ; Ectonucleotidase ; NTPDase ; Ecto-5′ ; -nucleotidase ; Zebrafish
• 刊名：Toxicology in Vitro
• 出版年：2006
• 出版时间：September 2006
• 年：2006
• 卷：20
• 期：6
• 页码：954-958
• 全文大小：245 K

文摘

Zinc and cadmium are environmental contaminants that induce a wide range of effects on CNS. Here we tested the in vitro effect of these metals on acetylcholinesterase (AChE) and ectonucleotidase (NTPDase and ecto-5′-nucleotidase) activities in zebrafish brain. Both zinc and cadmium treatments did not alter significantly the zebrafish brain AChE activity. ATP hydrolysis presented a significant increase at 1 mM zinc (17 % ) and the AMPase activity had a dose-dependent increase at 0.5 and 1 mM zinc exposure (188 % and 199 % ). After cadmium treatment, ATPase activity was significantly increased (53 % and 48 % ) at 0.5 and 1 mM, respectively. Cadmium, in the range 0.25–1 mM, inhibited ADP hydrolysis in a dose-dependent manner (13.4–69 % ). Ecto-5′-nucleotidase activity was only inhibited (38 % ) in the presence of 1 mM cadmium. It is possible to suggest that changes on NTPDase and ecto-5′-nucleotidase activities can be an important mechanism involved in neurotoxic effects promoted by zinc and cadmium.