Comparative sequence analysis of the SALT OVERLY SENSITIVE1

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• 作者：Gyoungju Nah ; Christopher L. Pagliarulo ; Peter G. Mohr ; Meizhong Luo ; Nick Sisneros ; Yeisoo Yu ; Kristi Collura ; Jennifer Currie ; Jose Luis Goicoechea ; Rod A. Wing ; Karen S. Schumaker
• 关键词：Thellungiella halophila ; SALT OVERLY SENSITIVE1 ; Salt tolerance ; Comparative sequence analysis ; Microcolinearity ; Simple sequence repeats
• 刊名：Genomics
• 出版年：2009
• 出版时间：September 2009
• 年：2009
• 卷：94
• 期：3
• 页码：196-203
• 全文大小：287 K

文摘

To provide a framework for studies to understand the contribution of SALT OVERLY SENSITIVE1 (SOS1) to salt tolerance in Thellungiella halophila, we sequenced and annotated a 193-kb T. halophila BAC containing a putative SOS1 locus (ThSOS1) and compared the sequence to the orthologous 146-kb region of the genome of its salt-sensitive relative, Arabidopsis thaliana. Overall, the two sequences were colinear, but three major expansion/contraction regions in T. halophila were found to contain five Long Terminal Repeat retrotransposons, MuDR DNA transposons and intergenic sequences that contribute to the 47.8-kb size variation in this region of the genome. Twenty-seven genes were annotated in the T. halophila BAC including the putative ThSOS1 locus. ThSOS1 shares gene structure and sequence with A. thaliana SOS1 including 11 predicted transmembrane domains and a cyclic nucleotide-binding domain; however, different patterns of Simple Sequence Repeats were found within a 540-bp region upstream of SOS1 in the two species.