The buckling and post-buckling behavior of a 3000 m subsea separator is analyzed by numerical and experimental methods. The nonlinear global buckling analysis is performed to analyze the global buckling behavior of the ultra-deep water subsea separator. In the experiment, a two circumferential lobe mode is present and the buckling deformation is asymmetrical. The nonlinear global buckling analysis proposed can numerically simulate the buckling process of the ultra-deep subsea separator with good accuracy.