Variations in genetic and chemical constituents of Ziziphus spina-christi L. populations grown at various altitudinal zonation up to 2227 m height

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• 作者：Mahmoud F. Moustafa^a ; ^b ; ^{mfmostfa@kku.edu.sa} ; Abd El-Latif Hesham^a ; ^c ; Manal S. Quraishi^a ; Sulaiman A. Alrumman^a
• 关键词：RAPD ; random amplified polymorphic DNA ; ISSR ; inter-simple sequence repeats markers ; GC&ndash ; MS ; gas chromatography&ndash ; mass spectrometry ; HPLC ; high performance liquid chromatography
• 刊名：Journal of Genetic Engineering and Biotechnology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：14
• 期：2
• 页码：349-362
• 全文大小：2625 K
• 卷排序：14

文摘

Altitudinal gradient-defined specific environmental conditions could lead to genetics and chemical variations among individuals of the same species. By using RAPD, ISSR, GC–MS and HPLC analysis, the genetic and chemical diversity of Ziziphus spina-christi plants at various altitudinal gradient namely; Abha (2227.86 m), Dala Valley (1424 m), Rakhma Valley (1000 m), Raheb Valley (505 m) and Al-Marbh (147 m) were estimated. RAPD markers revealed that the highest similarity value (40.22%) was between Raheb Valley and Al-Marbh while the lowest similarity (10.08%) was between Abha and Raheb Valley. Based on ISSR markers the highest similarity value (61.54%) was also between Raheb Valley and Al-Marbh, while the lowest similarity (26.84%) was between Abha and Rakhma Valley. GC–MS results showed the presence of various phytochemical constituents in each population. The dendrogram based on chemical compounds separated the Z. spina-christi grown at the highest elevations (Abha) from the populations in lower elevations. HPLC analysis showed that the leaves of Z. spina-christi plant contain considerable amount of vitamins including B1, B12, B2 and folic acid. In conclusion, there is a close relation between altitudinal gradients, genetic diversity and chemical constituents of the leaves of Z. spina-christi plants.