ISSR-based analysis of genetic diversity among sorghum landraces growing in some parts of Saudi Arabia and Yemen

详细信息    查看全文

• 作者：Mohammed Basahi ^{mbasahi@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Sorghum bicolor ; ISSR ; Genetic diversity ; DNA fingerprinting
• 刊名：Comptes Rendus Biologies
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：338
• 期：11
• 页码：723-727
• 全文大小：677 K

文摘

Inter simple sequence repeats (ISSR) analysis was used to determine the genetic diversity among 15 genotypes of sorghum [Sorghum bicolor (L.) Moench] growing in some parts of Saudi Arabia and Yemen. A total of 92 alleles were amplified, with an average of 13 ISSR alleles per primer. Cluster analysis divided the 15 genotypes into two main groups. Group A consisted of five genotypes with white grains from Jazan and Abha with a similarity coefficient range of 0.527 to 0.818. Group B was comprised of 10 genotypes; two genotypes from Al-Qassim were clearly delimited from the remaining eight samples with a coefficient range from 0.709 to 0.490. The eight genotypes were divided into two clusters; one was comprised of landraces with dark grains from Abha in Saudi Arabia and Ab in Yemen, with a similarity coefficient range between 0.563 and 0.781, and the other cluster was differentiated into three white-colored-grain genotypes and one colored-grain genotype; all samples from North Yemen had a similarity coefficient range from 0.454 to 0.800. The current results encourage further collection and authentication of sorghum landraces in the gene banks of Saudi Arabia.