Heparan sulfate/heparin glycosaminoglycan binding alters inhibitory profile and enhances anticoagulant function of conserved Amblyomma americanum tick saliva serpin 19

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• 作者：Željko M. Radulović ; Albert Mulenga ; ^{amulenga@cvm.tamu.edu}
• 关键词：Heparin ; Heparan sulfate ; A. americanum serpin 19 ; Tick blood feeding
• 刊名：Insect Biochemistry and Molecular Biology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：80
• 期：Complete
• 页码：1-10
• 全文大小：1604 K
• 卷排序：80

文摘

HSGAG binding enhances AAS19 inhibition of thrombin and factor IXa, but inhibits its activity against factors Xa and XIIa. HSGAG binding improved rAAS19 inhibitory activity 2.7–4.3 folds as determined by stoichiometry of inhibition analysis. rAAS19 in complex with HSGAGs dramatically delays plasma clotting time much more than rAAS19 or HSGAGs alone.