Functional characterization of naturally occurring melittin peptide isoforms in two honey bee species, Apis mellifera and Apis cerana

详细信息    查看全文

• 作者：Doori Park^a ; Je Won Jung^a ; Mi Ok Lee^c ; Si Young Lee^a ; Boyun Kim^a ; Hye Jun Jin^b ; Jiyoung Kim^a ; Young-Joon Ahn^a ; Ki Won Lee^a ; Yong Sang Song^a ; Seunghun Hong^b ; James E. Womack^c ; Hyung Wook Kwon^a ; ^{biomodeling@snu.ac.kr" class="auth_mail} ; ^{biomodels@gmail.com" class="auth_mail}
• 关键词：Antimicrobial peptide (AMP) ; Honey bee ; Single nucleotide polymorphism (SNP) ; Melittin ; Anti-inflammatory effect ; Anti-cancer activity
• 刊名：Peptides
• 出版年：March, 2014
• 年：2014
• 卷：53
• 期：Complete
• 页码：185-193
• 全文大小：2372 K

文摘

Insect-derived antimicrobial peptides (AMPs) have diverse effects on antimicrobial properties and pharmacological activities such as anti-inflammation and anticancer properties. Naturally occurring genetic polymorphism have a direct and/or indirect influence on pharmacological effect of AMPs, therefore information on single nucleotide polymorphism (SNP) occurring in natural AMPs provides an important clue to therapeutic applications. Here we identified nucleotide polymorphisms in melittin gene of honey bee populations, which is one of the potent AMP in bee venoms. We found that the novel SNP of melittin gene exists in these two honey bee species, Apis mellifera and Apis cerana. Nine polymorphisms were identified within the coding region of the melittin gene, of which one polymorphism that resulted in serine (Ser) to asparagine (Asp) substitution that can potentially effect on biological activities of melittin peptide. Serine-substituted melittin (Mel-S) showed more cytotoxic effect than asparagine-substituted melittin (Mel-N) against E. coli. Also, Mel-N and Mel-S had different inhibitory effects on the production of inflammatory factors such as IL-6 and TNF-伪 in BV-2 cells. Moreover, Mel-S showed stronger cytotoxic activities than Mel-N peptide against two human ovarian cancer cell lines. Using carbon nanotube-based transistor, we here characterized that Mel-S interacted with small unilamellar liposomes more strongly than Mel-N. Taken together, our present study demonstrates that there exist different characteristics of the gene frequency and the biological activities of the melittin peptide in two honey bee species, Apis mellifera and A. cerana.