Effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of Handmade cloned buffalo (Bubalus bubalis) embryos

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• 作者：S.K.  ; Panda ; A.  ; George ; A.  ; Saha ; R.  ; Sharma ; A.K.  ; Singh ; R.S.  ; Manik ; M.S.  ; Chauhan ; P.  ; Palta ; S.K.  ; Singla  ; ;   ; ^{singlasuresh@yahoo.com}
• 关键词：HMC ; Buffalo ; Parthenogenesis ; Scriptaid
• 刊名：Theriogenology
• 出版年：2012
• 出版时间：1 January 2012
• 年：2012
• 卷：77
• 期：1
• 页码：195-200
• 全文大小：680 K

文摘

The aim of the present study was to investigate the effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of Handmade cloned (HMC) and parthenogenetically activated (PA) buffalo embryos. Scriptaid treatment was given for 10 h immediately after reconstruction of HMC and PA embryos. Treatment of HMC embryos with 500 or 1000 nmol/L scriptaid significantly increased (P < 0.01) the cleavage rate (91.3 ¡À 2.8 and 91.9 ¡À 2.0 % , respectively) than that of controls (80.7 ¡À 2.4 % ). Blastocyst rate was significantly higher (P < 0.01) following treatment with 1000 nmol/L (54.1 ¡À 5.0 % ) than that with 500 nmol/L scriptaid (42.6 ¡À 2.9 % ) which, in turn, was higher (P < 0.01) than that of the controls (38.0 ¡À 2.6 % ). Also, HMC embryos treated with 500 or 1000 nmol/L scriptaid had higher (P < 0.01) cell number (339.9 ¡À 1.4 and 343.4 ¡À 2.4, respectively) than the untreated embryos (150.7 ¡À 2.0). In contrast, in zona-free PA embryos, the cleavage and blastocyst rate was significantly lower (P < 0.01) following treatment with 500 (46.3 ¡À 1.0 and 22.0 ¡À 1.4 % , respectively) or 1000 nmol/L scriptaid (46.2 ¡À 1.9 and 16.6 ¡À 1.3 % , respectively) than that of untreated embryos (60.4 ¡À 3.2 and 29.5 ¡À 2.1 % , respectively). Transfer of HMC embryos that had been untreated (N = 13) or treated with 1000 nmol/L scriptaid (N = 15) to synchronized recipients resulted in one pregnancy each. In conclusion, these results demonstrate that scriptaid treatment improves the developmental potential of HMC buffalo embryos, but compromises that of zona-free PA embryos.