Functional analysis of ¦Á1,3/4-fucosyltransferase VI in human hepatocellular carcinoma cells

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• 作者：Qiya Guo ; Bin Guo ; Yingming Wang ; Jun Wu ; Wenjun Jiang ; Shenan Zhao ; Shouyi Qiao ; ^{syqiao@fudan.edu.cn} ; Yanhua Wu ; ^{yanhuawu@fudan.edu.cn}
• 关键词：Fucosyltransferase (FUT) family ; Hepatocellular carcinoma (HCC) ; Phosphatidyl inositol 3-kinase/Akt (PI3K/Akt) ; p21
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 出版时间：6 January 2012
• 年：2012
• 卷：417
• 期：1
• 页码：311-317
• 全文大小：896 K

文摘

The ¦Á1,3/4-fucosyltransferases (FUT) subfamily are key enzymes in cell surface antigen synthesis during various biological processes. A novel role of FUTs in tumorigenesis has been discovered recently, however, the underlying mechanism remains largely unknown. Here, we characterized FUT6, a member of ¦Á1,3/4-FUT subfamily, in human hepatocellular carcinoma (HCC). In HCC tissues, the expression levels of FUT6 and its catalytic product SLe^x were significantly up-regulated. Overexpression of FUT6 in HCC cells enhanced S-phase cell population, promoted cell growth and colony formation ability. Moreover, subcutaneously injection of FUT6-overexpressing cells in nude mice promoted cell growth in vivo. In addition, elevating FUT6 expression markedly induced intracellular Akt phosphorylation, and suppressed the expression of the cyclin-dependent kinases inhibitor p21. Bath application of the PI3K inhibitor blocked FUT6-induced Akt phosphorylation, p21 suppression and cell proliferation. Our results suggest that FUT6 plays an important role in HCC growth by regulating the PI3K/Akt signaling pathway.