A rapid test for the detection of influenza A virus including pandemic influenza A/H1N1 2009

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• 作者：Yixin Chen ; Feihai Xu ; Xun Gui ; Kunyu Yang ; Xingrong Wu ; Qingbing Zheng ; Shengxiang Ge ; Quan Yuan ; Anthony E.T. Yeo ; Jun Zhang ; Yi Guan ; Honglin Chen ; Ningshao Xia
• 关键词：Influenza A ; Nucleoprotein ; Rapid test ; Dot-ELISA
• 刊名：Journal of Virological Methods
• 出版年：2010
• 出版时间：July 2010
• 年：2010
• 卷：167
• 期：1
• 页码：100-102
• 全文大小：116 K

文摘

A new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; third, the kit was tested against specimens collected in the field obtained from human and chicken; and fourth, the kit was tested against the novel pandemic influenza A/H1N1 2009 clinical specimens obtained from admitted to hospital patients. The test kit displayed a sensitivity of 88 % for both human specimens and avian specimens. The corresponding specificity was 99.3 % for human specimens and 96.5 % for avian specimens. This test kit may be useful for rapid diagnosis of influenza A virus.