Upconversion nanoparticles for differential imaging of plant cells and detection of fluorescent dyes

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• 作者：Xiaofeng WU ((吴笑峰))^a ; Pan HU ((胡 盼))^a ; Shigang HU ((胡仕刚))^a ; Zenghui CHEN ((陈增辉))^b ; Huanyuan YAN ((严焕元))^a ; ^c ; Zhijun TANG ((唐志军))^a ; Zaifang XI ((席在芳))^a ; Yi YU ((余 意))^a ; Gangtao DAI ((戴港涛))^b ; Yunxin LIU ((刘云新))^b ; ^d ; ^{lyunxin@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：upconversion ; luminescence ; fluorescent dye ; cell imaging ; detection ; rare earths
• 刊名：Journal of Rare Earths
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：34
• 期：2
• 页码：208-220
• 全文大小：2761 K

文摘

Upconversion NaLuF₄ nanoparticles were synthesized by the solvothermal method which could emit multicolor visible light under the excitation of 980 nm near-infrared (NIR) photons. These upconversion nanoparticles (UCNPs) with an acidic ligand could rapidly capture the basic rhodamine-B (RB) in plant cells to generate a close UCNPs@RB system. RB could efficiently absorb the green fluorescence from NaLuF₄:18 mol.%Yb³⁺,2 mol.%Er³⁺ UCNPs and then emitted red light in the UCNPs@RB system by a robust luminescence resonance energy transfer (LRET) from UCNPs to RB. The detection limit of RB with these upconversion fluorescent nanoprobes could reach 0.25 μg/cm³ in plant cell even under an ultra low excitation power source of 0.2 W/mm². This LRET phenomenon was also extended to NaLuF₄:18 mol.%Yb³⁺,0.5 mol.%Tm³⁺@Sodium fluorescein (SF) system. In addition, the differential imaging could be achieved by successively incubating plant cells with fluorescent dyes and UCNPs. The fluorescent dyes aggregated in cell wall while UCNPs with surface modification distributed both in cell wall and cytoplasm, so that UCNPs@Dyes formed in cell walls which could emit multicolor light by LRET which was different from the emission in cytoplasm with only UCNPs.