Visual and multiplex detection of nucleic acid sequence by multiple cross displacement amplification coupled with gold nanoparticle-based lateral flow biosensor
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- 作者：Yi WangAuthor Vitae ; Yan WangAuthor Vitae ; Lu ZhangAuthor Vitae ; Jianguo Xu ; xujianguo@icdc.cn (Prof.)Author Vitae ; Changyun Ye ; yechangyun@icdc.cn (Prof.)Author Vitae
- 关键词：MCDA ; LFB ; MCDA-LFB ; LoD ; Salmonella spp. ; Shigella spp.
- 刊名：Sensors and Actuators B: Chemical
- 出版年：2017
- 出版时间：31 March 2017
- 年：2017
- 卷：241
- 期：Complete
- 页码：1283-1293
- 全文大小：3728 K
- 卷排序：241
文摘
A novel biosensor based on multiple cross displacement amplification and lateral flow technique for visual detection of nucleic acids was devised. The nearly apparatus-free platform of the assay make it practical for point-of-care testing, field detection, ‘on-site’ diagnosis and more. The assay displayed unique advantages of simplicity, rapidity, sensitivity, specificity and repeatability.