Ethanol production from acid hydrolysate of wood biomass using the flocculating yeast Saccharomyces cerevisiae strain KF-7

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• 作者：Yueqin Tang ; Minzhe An ; Kai Liu ; Saki Nagai ; Toru Shigematsu ; Shigeru Morimura ; Kenji Kida
• 关键词：Fuel ethanol production ; Wood biomass ; Continuous ethanol fermentation ; Flocculating yeast
• 刊名：Process Biochemistry
• 出版年：2006
• 出版时间：April 2006
• 年：2006
• 卷：41
• 期：4
• 页码：909-914
• 全文大小：308 K

文摘

An ethanol production process using the flocculating yeast Saccharomyces cerevisiae strain KF-7 with acid hydrolysate of wood biomass as feed was investigated. Corn steep liquor (CSL) supplemented with KH₂PO₄, MgSO₄, and CaCl₂ was used as the source of nitrogen and mineral nutrients. Using a tower-type reactor (working volume (WV), 0.45 L), continuous fermentation with a high ethanol productivity of over 20 g/(L h) was achieved at a dilution rate of 0.3 h⁻¹ with a pH of 4.5 at 35 °C. A continuous fermentation process using two tower-type reactors (WV, 4.5 L each) connected in series was then constructed to decrease the concentration of residual sugars (except for xylose). The contamination of bacteria was effectively repressed and an ethanol productivity of over 12.6 g/(L h) was achieved at an overall dilution rate of 0.2 h⁻¹ with a pH of 4.0 at 35 °C. Strain KF-7 was thus demonstrated to be suitable for the production of ethanol from acid hydrolysate of wood biomass.