Antidepressant-like effects of water extract of Gastrodia elata Blume in rats exposed to unpredictable chronic mild stress via modulation of monoamine regulatory pathways

详细信息    查看全文

• 作者：Yu-En Lin^a ; Shih-Hang Lin^a ; Wei-Cheng Chen^a ; Chi-Tang Ho^b ; Yi-Syuan Lai^a ; Suraphan Panyod^a ; Lee-Yan Sheen^a ; ^{lysheen@ntu.edu.tw" class="auth_mail" title="E-mail the corresponding author}
• 关键词：WGE ; water extract of Gastrodia elata Blume ; GAS ; gastrodin ; UCMS ; unpredictable chronic mild stress ; MAO ; monoamine oxidase ; 5-HT ; 5-hydroxytryptophan (serotonin) ; 5-HIAA ; 5-hydroxyindoleacetic acid ; DA ; dopamine ; DOPAC ; 3 ; 4-dihydroxyphenylacetic acid ; HVA ; homovanillic acid ; PC12 ; pheochromocytoma cells ; TH ; tyrosine hydroxylase ; CTL ; control ; CW ; control plus WGE treatment ; UW ; UCMS plus WGE treatment
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：1 July 2016
• 年：2016
• 卷：187
• 期：Complete
• 页码：57-65
• 全文大小：1201 K

文摘

Gastrodia elata Blume (GE) is a traditional herbal medicine belonging to the Orchidaceae family, and has been used to manage neurological disorders for centuries. We have previously reported that its water extract (WGE) could improve the depressive-like behaviours in the forced swimming test (FST), an animal model of depression.

Aim of the study

To investigate the antidepressant-like effects of WGE in rats exposed to unpredictable chronic mild stress (UCMS) model, and to explore its possible molecular mechanisms.

Materials and methods

UCMS rats were orally administered with WGE (0.5 g/kg body weight) daily within the 4 weeks UCMS procedure. The sucrose preference test and the open field test were conducted to assess anhedonia and spontaneous behaviours, respectively. The cerebral turnover rates of monoamine neurotransmitters and the serum corticosterone levels were measured. In vitro direct and indirect monoamine oxidase A (MAO-A) inhibitory assays were employed to assess the possible antidepressant-like mechanisms of WGE (0.5 mg/mL) and its major component, gastrodin (GAS, 15, 30 and 60 μg/mL). Western blot was used to examine the expression of protein related to monoamine regulation, such as MAO-A and tyrosine hydroxylase (TH).

Results

WGE significantly reversed the sucrose preference and other abnormal behaviours induced by 4 weeks of UCMS. WGE significantly restored the cerebral turnover rates of serotonin and dopamine and decreased serum corticosterone levels. WGE and gastrodin inhibited the activity and protein expression of MAO-A, and increased TH levels in PC12 cells.

Conclusion

The antidepressant-like effects of WGE and gastrodin might be mediated by the regulation of monoamine neurotransmitters, and therefore were beneficial in depression treatment as a complementary approach.