Micellar electrokinetic chromatography with polyelectrolyte complexes as micellar pseudo-stationary phases
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- 作者：Shpak ; Alexey V. ; Pirogov ; Andrey V. ; Shpigun ; Oleg A.
- 关键词：Pseudo-stationary phases ; Micellar electrokinetic chromatography ; Polyelectrolyte complexes ; Polyacrylic acid ; Amino acids
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2004
- 出版时间：February 5, 2004
- 年：2004
- 卷：800
- 期：1-2
- 页码：91-100
- 全文大小：224 K
文摘
The separation of dansyl (DNS-AAs) and carbobenzoxy (CBZ-AAs) amino acids using micellar electrokinetic chromatography employing polyelectrolyte-surfactant complexes (PSC) formed in the reaction between polyacrylic acid (PAA) and dodecyltrimethylammonium bromide (DTAB) as pseudo-stationary phases was described. The PSCs were stabilized by hydrophobic interactions of alkyl chains of the surfactant ions and converted to an intramolecular micellar-like phase. The running buffer was a 50mM solution of sodium phosphate (pH 6.0) containing 4.6–20.2mM PSC, in which a part of carboxyl groups of PAA was blocked by aliphatic amines. For the systems with 7.9mM of PAA/DTAB complex (