Ultrasensitive electrochemical sensing platform for microRNA based on tungsten oxide-graphene composites coupling with catalyzed hairpin assembly target recycling and enzyme signal amplification
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- 作者：Hong-Lei Shuai ; Ke-Jing Huang ; kejinghuang@163.com" class="auth_mail" title="E-mail the corresponding author ; Ling-Li Xing ; Ying-Xu Chen
- 关键词：Tungsten oxide-graphene ; Target recycling ; miRNAs biosensing ; Catalyzed hairpin assembly ; Electrochemical-chemical-chemical redox cycling
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：337-345
- 全文大小：1845 K
文摘
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A hydrothermal approach is used for the synthesis tungsten oxide-graphene composites.
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A miRNA sensor is developed based on catalyzed hairpin assembly target recycling.
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Electrochemical-chemical-chemical system was applied to amply detection signal.
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The biosensor shows a detection limit of 0.05 fM and high specificity towards miRNA.