Caffeine Increases Tear Volume Depending on Polymorphisms within the Adenosine A2a Receptor Gene and Cytochrome P450 1A2

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• 作者：Reiko Arita ; MD ; PhD¹ ; ² ; ^{ritoh@za2.so-net.ne.jp} ; Yasuo Yanagi ; MD ; PhD² ; Norihiko Honda ; MD ; PhD² ; Shuji Maeda ; MD ; PhD³ ; Koshi Maeda ; MD ; PhD³ ; Aya Kuchiba ; PhD⁴ ; Takuhiro Yamaguchi ; PhD⁵ ; Yoshitsugu Yanagihara ; PhD⁶ ; Hiroshi Suzuki ; MD ; PhD⁶ ; Shiro Amano ; MD ; PhD²
• 刊名：Ophthalmology
• 出版年：2012
• 出版时间：May, 2012
• 年：2012
• 卷：119
• 期：5
• 页码：972-978
• 全文大小：264 K

文摘

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Purpose

The primary aim of the present study was to examine the effect of caffeine on tear volume. The secondary aim was to investigate the relation between caffeine-induced changes in tear volume and polymorphisms in ADORA2A and CYP1A2.

Design

Double-masked, placebo-controlled, crossover study.

Participants

Seventy-eight healthy volunteers were recruited for the study.

Methods

Subjects participated in 2 sessions in which they received capsules containing either placebo or caffeine. The caffeine capsules were given to the subjects to keep the caffeine volume per body weight within 5 to 7 mg/kg. After caffeine intake, tear meniscus height (TMH) was measured. Subjects provided a blood sample for genotyping.

Main Outcome Measures

Tear meniscus height, single nucleotide polymorphism.

Results

The tear volume increased after caffeine consumption. The net increase in TMH was 0.08 mm (95 % confidence interval, 0.05-0.10) greater when participants were given caffeine than when given placebo (P<0.0001). In ADORA2A, the difference in the net increase in TMH for participants who were heterozygous at rs5751876 and rs2298383 was 0.07 mm (P = 0.001) and who were minor homozygous was 0.08 mm (P = 0.007). In CYP1A2, the net increase in TMH for participants who were minor homozygous at rs2472304 was lower than for those who were major homozygous; the difference was 0.06 mm (P = 0.039).

Conclusions

Caffeine intake increases tear volume and polymorphisms within ADORA2A, and CYP1A2 is associated with the tear increase after caffeine intake. Genetic polymorphisms had a significant effect on tear meniscus that was of limited clinical significance.

Financial Disclosure(s)

The authors have no proprietary or commercial interest in any of the materials discussed in this article.