Histopathological lesions in encephalon and heart of mice infected with Toxoplasma gondii increase after Lycopodium clavatum 200dH treatment

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• 作者：Andr&eacute ; ia Vieira Pereira^a ; Marcelo Biondaro G&oacute ; is^b ; ^{marcelobiondaro@gmail.com} ; Katiucha Rebeca Jennifer Lopes Lera^c ; Gislane Janaina Falkowski-Temporini^d ; Paula Fernanda Massini^d ; Ricardo Nascimento Drozino^d ; Denise Lessa Aleixo^d ; Milena Menegazzo Miranda^a ; Paulo da Silva Watanabe^b ; Ivete Conchon-Costa^a ; Idessania Nazareth da Costa^a ; M&aacute ; rio dos Anjos Neto Filho^c ; Silvana Marques de Ara&uacute ; jo^d ; Wander Rog&eacute ; rio Pavanelli^a
• 关键词：Toxoplasmosis ; Myocarditis ; Encephalitis ; Inflammatory infiltrates
• 刊名：Pathology - Research and Practice
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：213
• 期：1
• 页码：50-57
• 全文大小：2389 K
• 卷排序：213

文摘

In many cases, symptoms of toxoplasmosis are mistaken for the ones of other infectious diseases. Clinical signs are rare in immunocompetent people. However, when they arise, in the acute phase of infection, several organs are affected due to the rapid spread of tachyzoites through the bloodstream. In the present study, the reduction of tachyzoites in peripheral blood of mice of G72 (infected 72 h after treatment) and G48 (infected 48 h after treatment and treated three more times), when compared with IC (infected and non-treated), suggests protective effect exerted by Lycopodium clavatum. If on the one hand L. clavatum brought benefits, reducing parasitemia, on the other hand, the parasitism became exacerbated. Histopathological analysis demonstrated focal, multifocal and diffuse inflammatory infiltrates, ranging from absent, discreet, moderate to intense, in heart and encephalon of mice of NIC (non-infected and non-treated), IC, G48 and G72 groups, respectively. In the perivascular region and meninges, the injuries were enlarged. The presence of tachyzoites was demonstrated through immunohistochemical (IHC) assay in myocardium. Toxoplasma gondii induced increase of collagen fibers in myocardium of mice of G72 and G48 groups, compared with IC (p < 0.05) and NIC (p < 0.001). The presence of inflammatory infiltrates, as well as the progressive fibrosis, caused myocardial remodeling in animals treated with L. clavatum. Counterstaining with H&E suggests TGF-β expression by mononuclear cells in the inflammatory infiltrate. Based on our results, we can conclude that the adopted regimen and potency exerted a protective effect, reducing parasitemia. However, it intensified the histopathological lesions in encephalon and heart of mice infected with T. gondii.