RAPD and morphological diversity among four populations of the tropical tree species Paramichelia baillonii (Pierre) Hu in China

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• 作者：Fagen Li ; Siming Gan ; Qijie Weng ; Xia Zhao ; Shineng Huang ; Mei Li ; Shaoyu Chen ; Qinghua Wang ; Fuqiang Shi
• 关键词：Paramichelia baillonii ; Genetic variation ; Molecular marker ; Phenotype ; Conservation ; Domestication
• 刊名：Forest Ecology and Management
• 出版年：2008
• 出版时间：5 April 2008
• 年：2008
• 卷：255
• 期：5-6
• 页码：1793-1801
• 全文大小：397 K

文摘

Genetic variation and structure of four natural populations of Paramichelia baillonii (Pierre) Hu (family Magnoliaceae) from China were assessed using random amplified polymorphic DNA (RAPD) markers and morphological characters. For RAPD analysis, 17 primers generated a total of 152 discernible and reproducible bands across the four populations analyzed, out of which 63 (41.4 % ) were polymorphic. The Shannon's index (H_o) varied with population, ranging from 0.555 to 1.121, with an average value of 0.955. The mean diversity at species level was H_sp = 1.217. Most of the genetic variation was found within population although pair-wise Φ_ST values and Bartlett's test indicated significant differentiation among populations. The between-population component comprised 21.1 % and 15.7 % of the total genetic variation, respectively, as assessed by the Shannon's index and AMOVA analysis. The gene flow (N_m) ranged from a moderate value of 0.464 (between populations YL and YE) to a high value of 4.128 (between populations YP and YE), with an average value of 1.570. The results for P. baillonii are generally in accordance with those already recorded for other outcrossing tropical tree species. For morphological characters, principal component analysis showed that the first two components explained a total of 77.6 % of the whole phenotypic variability, but a dispersion plot of all the families defined by the first two principal components did not show any significant grouping. The overall population differentiation index in quantitative traits (Q_ST) is 0.07 and less than that at RAPD markers (Φ_ST, 0.157). The pair-wise Q_ST estimates are significantly correlated with pair-wise Φ_ST (r = 0.8643, P = 0.0264) while the correlation between the Euclidean distance matrices based on morphological and RAPD data was low and non-significant (r = −0.0189, P = 0.3103). The information obtained here could be valuable for devising strategies for conservation and domestication of P. baillonii.