Establishment of the complete life cycle of Spirometra (Cestoda: Diphyllobothriidae) in the laboratory using a newly isolated triploid clone

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• 作者：Tetsuya Okino^a ; ^{okino@med.kawasaki-m.ac.jp} ; Hiroshi Ushirogawa^a ; Kumiko Matoba^a ; Shin-ichiro Nishimatsu^b ; Mineki Saito^a ; ^{mineki@med.kawasaki-m.ac.jp}
• 关键词：Triploid ; Spirometra erinaceieuropaei ; Complete life cycle ; Sparganosis ; New model organism
• 刊名：Parasitology International
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：66
• 期：2
• 页码：116-118
• 全文大小：536 K
• 卷排序：66

文摘

Methods to maintain the life cycle of pathogenic organisms become powerful tools for studying molecular and cellular bases of infectious diseases. Spirometra erinaceieuropaei is a parasitic tapeworm that causes sparganosis in humans. Because S. erinaceieuropaei has a complex life cycle with different stages and host species requirements, there have been no reports to establish the complete life cycle in the laboratory. In this study, using Cyclops as the first intermediate host, mouse as the experimental second intermediate host, and dog as the final host, we succeeded in maintaining S. erinaceieuropaei in the laboratory. By repeating the established life cycle five times, we obtained a clonal population of S. erinaceieuropaei from a single adult worm. A karyotype study showed that the chromosome of this clone is triploid (3n = 27), indicating that a genetically uniform strain is established by apomictic reproduction. The strain was named Kawasaki triploid (Kt). A partial sequence of mitochondrial cytochrome c oxidase subunit 1 gene of the strain Kt showed more than 98% similarity with those of S. erinaceieuropaei isolates from Australia, China, and South Korea, and the resultant phylogeny indicated that the strain Kt is a member of a distinctive clade from East Asia and Oceania. Our system will be particularly useful for studies of S. erinaceieuropaei infection and human sparganosis.