MiR-595 targeting regulation of SOX7 expression promoted cell proliferation of human glioblastoma

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• 作者：Yu Hao^a ; Shubao Zhang^b ; Shaojun Sun^a ; ^{shaojunsun2011@163.com" class="auth_mail" title="E-mail the corresponding author} ; Jianxin Zhu^b ; Yilei Xiao^b
• 关键词：miR-595 ; Glioblastoma ; SOX7 ; Cell proliferation
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：80
• 期：Complete
• 页码：121-126
• 全文大小：1611 K

文摘

Increasing evidence indicated that dysregulation of microRNAs (miRNAs) were involved with human disease including cancer. Recently, miR-595 was reported as a tumor promoter in malignant mesothelioma. However, the underlying mechanism of miR-595 in human glioblastoma (GBM) cells have not been well elucidated. Therefore, in this study, we investigated the biological functions and molecular mechanisms of miR-595 in human GBM. MiR-595 expression was significantly upregulated in GBM tissues and cells. We modified miR-595 levels in GBM cells and investigated their effects on the cell proliferation by MTT, colony formation and anchorage-independent growth assays. We found that miR-595 significantly increased GBM cell proliferation. Bioinformatic analysis predicted that miR-595 may target the 3′-UTR of SOX7and suppressed its translation, and further confirmed by luciferase assay. In sum, these observations together indicated that miR-595 played a critical role in carcinogenesis by suppression of SOX7, and may serve as a therapeutic target for the treatment of GBM.