A non-invasive method for studying viral DNA delivery to bacteria reveals key requirements for phage SPP1 DNA entry in Bacillus subtilis cells
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- 作者：Sofia Fernandesa ; Audrey Labardeb ; c ; Catarina Baptistaa ; Lina Jakutytè ; c ; 1 ; Paulo Tavaresb ; c ; Carlos Sã ; o-José ; a ; csaojose@ff.ul.pt" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bacteriophage ; SPP1 ; B. subtilis ; Virus entry ; Membrane potential ; Calcium ; YueB
- 刊名：Virology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：495
- 期：Complete
- 页码：79-91
- 全文大小：1553 K
文摘
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A new method to study bacteriophage DNA delivery to host cells was developed.
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The method has the potential to be used with many tailed bacteriophages.
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Its application revealed that an energized B. subtilis membrane is crucial for phage SPP1 DNA entry.