Direct Evidence for Packaging Signal-Mediated Assembly of Bacteriophage MS2
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- 作者：Ó ; ttar Rolfsson1 ; 5 ; &dagger ; ; Stefani Middleton2 ; 3 ; &dagger ; ; Iain W. Manfield1 ; Simon J. White1 ; Baochang Fan2 ; Robert Vaughan2 ; Neil A. Ranson1 ; Eric Dykeman4 ; Reidun Twarock4 ; James Ford3 ; C. Cheng Kao2 ; Peter G. Stockley1 ;
- 关键词：CP ; coat protein ; MP ; maturation protein ; PS ; packaging signal ; ssRNA ; single-stranded RNA ; EM ; electron microscopy ; EDTA ; ethylenediaminetetraacetic acid
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：29 January 2016
- 年：2016
- 卷：428
- 期：2
- 页码：431-448
- 全文大小：2642 K
文摘
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CLIP-Seq reveals a defined set of RNA–coat interactions in a single-stranded RNA virion.
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RNA sites match previous predictions of multiple PSs.
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Data provide unambiguous confirmation of PS-mediated assembly.