Monoclonal antibodies targeting ST2L Domain 1 or Domain 3 differentially modulate IL-33-induced cytokine release by human mast cell and basophilic cell lines
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- 作者：Natalie Fursova ; Jin Lua ; 1 ; Catherine Healyb ; Sheng-Jiun Wua ; Eilyn Lacya ; Angela Filerb ; Yawei Lib ; Changbao Liub ; Roberta Lambb ; Brian Jonesb ; Ramachandra Reddya ; Ted Petleya ; Karen Duffyb ; kduffy@its.jnj.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Fab ; antigen-binding fragment of antibody ; HRP ; horseradish peroxidase ; Ig ; immunoglobulin ; IL-33 ; interleukin 33 ; IL-1RAcP ; interleukin 1 receptor accessory protein ; mAb ; monoclonal antibody ; MAPK ; mitogen activated protein kinase ; mRNA ; messenger RNA ; NF-κB ; nuclear factor κB ; PBS ; phosphate-buffered saline ; SCF ; stem cell factor ; sST2 ; soluble form of ST2 ; ST2L ; long form of ST2
- 刊名：Molecular Immunology
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：75
- 期：Complete
- 页码：178-187
- 全文大小：2032 K
文摘
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Monoclonal antibodies targeting Domain 1 or Domain3 of the IL-33 receptor ST2L differentially affect signaling complex formation.
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The anti-ST2L antibodies differentially affect gene expression and cytokine release by mast cells as compared to basophils.
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Blocking the first step in the formation of the ST2L signaling complex with an anti-ST2L Domain 1 mAb resulted in complete inhibition.
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Partial inhibition of the pathway as well as aberrant signaling was observed in mast cells treated with an anti-ST2L Domain 3 mAb.