Reliable and durable Golgi staining of brain tissue from human autopsies and experimental animals
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- 作者：Gorazd B. Rosoklijaa ; b ; d ; gbr2@columbia.edu" class="auth_mail ; Vladimir M. Petrushevskie ; Aleksandar Stankovc ; Ani Dikac ; Zlatko Jakovskic ; Goran Pavlovskic ; Natasha Davchevac ; Richard Lipkina ; Tatiana Schniedera ; Kimberley Scobief ; Aleksej Dumac ; Andrew J. Dworka ; b ; d
- 关键词：Golgi-Kopsch ; Rapid Golgi ; Golgi-Cox ; Autopsy ; Human ; Experimental animals ; Dendrites ; Dendritic spines
- 刊名：Journal of Neuroscience Methods
- 出版年：15 June 2014
- 年：2014
- 卷：230
- 期：Complete
- 页码：20-29
- 全文大小：6299 K
文摘
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A reliable procedure for Golgi staining of cerebral cortical neurons is described.
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Impregnated cells are uniformly distributed, and all depths of the processed tissue block are usable for observational and quantitative morphometric analysis.
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All stained cells appear to be completely impregnated.
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Staining lasts at least 8 years and is apparently permanent.
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The procedure must start with unfixed tissue and requires approximately 4 months for completion.