The protection of bioenergetic functions in mitochondria by new synthetic chromanols

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• 作者：Katrin Staniek ; Thomas Rosenau ; Wolfgang Gregor ; Hans Nohl and Lars Gille
• 关键词：Mitochondria ; Bioenergetics ; Lipid peroxidation ; Thiol oxidation ; Chromanols ; Tocopheryl quinone
• 刊名：Biochemical Pharmacology
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：70
• 期：9
• 页码：1361-1370
• 全文大小：257 K

文摘

α-Tocopherol is the most important lipophilic antioxidant of the chromanol type protecting biomembranes from lipid peroxidation (LPO). Therefore, α-tocopherol and its derivatives are frequently used in the therapy or prevention of oxygen radical-derived diseases. In the present study, novel chromanol-type antioxidants (twin-chromanol, cis- and trans-oxachromanol) as well as the well-known short-chain analogue of α-tocopherol, pentamethyl-chromanol, were tested for their antioxidative potency in rat heart mitochondria (RHM). Our experiments revealed that the bioenergetic parameters of mitochondria were not deteriorated in the presence of chromanols (up to 50 nmol/mg protein). Exposure of RHM to cumene hydroperoxide and Fe²⁺ (final concentrations 50 μM each), inducing LPO, significantly affected their bioenergetic parameters which were determined in the presence of glutamate and malate (substrates of mitochondrial complex I). Alterations of the bioenergetic parameters were partially prevented in a concentration-dependent manner by preincubating RHM with antioxidants before adding the radical-generating system. In the lower concentration range, twin-chromanol turned out to be more efficient than pentamethyl-chromanol, both being far more protective than cis- and trans-oxachromanol. Measurement of protein-bound SH groups and thiobarbituric acid-reactive substances revealed that this protective effect was due to their antioxidative action. Furthermore, HPLC measurements of α-tocopherol and α-tocopheryl quinone in rat liver mitochondria demonstrated an α-tocopherol-sparing effect of twin-chromanol. In conclusion, new chromanol-type antioxidants, especially twin-chromanol, were able to improve bioenergetic and biochemical parameters of mitochondria exposed to oxidative stress.