Structural analysis of a fucoidan from the brown alga Fucus evanescens

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• 作者：Stanislav D. Anastyuk ; Natalia M. Shevchenko ; Evgeny L. Nazarenko ; Pavel S. Dmitrenok ; Tatyana N. Zvyagintseva
• 关键词：Fucoidan ; Sulfated polysaccharide ; Structure ; ESIMS ; MALDI-TOFMS ; Brown alga
• 刊名：Carbohydrate Research
• 出版年：2009
• 出版时间：21 April 2009
• 年：2009
• 卷：344
• 期：6
• 页码：779-787
• 全文大小：979 K

文摘

A fucoidan, a heterogeneous sulfated polysaccharide from the brown alga Fucus evanescens, was depolymerized under solvolytic conditions, and its ethanol-extracted low-molecular-weight fraction was analyzed by MALDI-TOFMS and ESIMS/MS. It was found that the mixture contained unsulfated oligosaccharides including some monosulfated components, which were shown to consist of mainly (1→3)-linked 2-O-sulfonated fucose residues (from 1 to 4). Minor components of the mixture were shown to contain 2-O- and 4-O-sulfonated xylose and galactose residues. Among them, mixed monosulfonated fucooligosaccharides were detected and characterized: Xyl-(1→4)-Fuc, Gal-(1→4)-Fuc, Gal-(1→4)-Gal-(1→4)-Fuc, Gal-(1→4)-Gal. Fucose, galactose, and xylose residues were shown to be mainly 2-O-sulfonated with traces of 4-O-sulfonation. Glucuronic acid was also found as a part of non-sulfated fucooligosaccharides: Fuc-(1→3)-GlcA, Fuc-(1→4)-Fuc-(1→3)-GlcA, Fuc-(1→3)-Fuc-(1→4)-Fuc-(1→3)-GlcA.