α_2A-adrenoceptors, but not nitric oxide, mediate the peripheral cardiac sympatho-inhibition of moxonidine

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• 作者：Luis E. Cobos-Puc^a ; ^{luis.cobos@uadec.edu.mx" class="auth_mail" title="E-mail the corresponding author} ; Hilda Aguayo-Morales^b ; Yesenia Silva-Belmares^a ; Maria A. Gonzá ; lez-Zavala^a ; David Centurió ; n^b
• 关键词：Moxonidine hydrochloride (PubChem CID ; 11231255) ; BRL 44408 maleate salt (PubChem CID ; 71311805) ; imiloxan hydrochloride (PudChem CID ; 172975) ; JP-1302 dihydrochloride (PubChem CID ; 540335) ; AGN 192403 hydrochloride (PubChem CID ; 11957452) ; L-NAME hydrochloride (PubChem CID ; 135193)
• 刊名：European Journal of Pharmacology
• 出版年：2016
• 出版时间：5 July 2016
• 年：2016
• 卷：782
• 期：Complete
• 页码：35-43
• 全文大小：1133 K

文摘

Moxonidine centrally inhibits the sympathetic activity through the I₁-imidazoline receptor and nitric oxide. In addition, inhibits the peripheral cardiac sympathetic outflow by α₂-adrenoceptors/I₁-imidazoline receptors, although the role of α₂-adrenoceptor subtypes or nitric oxide in the cardiac sympatho-inhibition induced by moxonidine are unknown. Therefore, the cardiac sympatho-inhibition induced by moxonidine (10 μg/kg min) was evaluated before and after of the treatment with the following antagonists/inhibitor: (1) BRL 44408, (300 μg/kg, α_2A), imiloxan, (3000 μg/kg, α_2B), and JP-1302, (300 μg/kg, α_2C), in animals pretreated with AGN 192403 (3000 μg/kg, I₁ antagonist); (2) N^ω-nitro-l-arginine methyl ester (l-NAME; 34, 100, and 340 μg/kg min); and (3) the combinations of the highest dose of l-NAME plus AGN 192403 or BRL 44408. Additionally, the expression of the neuronal (nNOS) and inducible (iNOS) nitric oxide synthase in the stellate ganglion was determined after treatment with moxonidine (i.p. 0.56 mg/kg daily, during one week). The cardiac sympatho-inhibition of 10 μg/kg min moxonidine was: (1) unaffected by imiloxan and JP-1302, under pretreatment with AGN 192403, or l-NAME (34, 100 and 340 μg/kg min) given alone; (2) partially antagonized by the combination of 340 μg/kg min l-NAME plus BRL 44408; and (3) abolished by BRL 44408 under treatment with AGN 192403. Furthermore, moxonidine did not modify the nNOS or iNOS protein expression in the stellate ganglion, the main source of postganglionic sympathetic neurons innervating the heart. In conclusion, our results suggest that the peripheral cardiac sympatho-inhibition induced by moxonidine is mediated by α_2A-adrenoceptor subtype but not by nitric oxide.