Photoinduced reduction of the medial FeS center in the hydrogenase small subunit HupS from Nostoc punctiforme
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- 作者：Patrí ; cia Raleiras ; Leif Hammarströ ; m ; Peter Lindblad ; Stenbjö ; rn Styring ; stenbjorn.styring@kemi.uu" class="auth_mail" title="E-mail the corresponding author ; Ann Magnuson ; ann.magnuson@kemi.uu.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：Hydrogenase ; Iron&ndash ; sulfur ; Electron transfer ; Flash photolysis ; Electron paramagnetic resonance
- 刊名：Journal of Inorganic Biochemistry
- 出版年：2015
- 出版时间：July 2015
- 年：2015
- 卷：148
- 期：Complete
- 页码：57-61
- 全文大小：379 K
文摘
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The small subunit, HupS, from a cyanobacterial uptake hydrogenase was expressed and isolated.
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HupS was subject to flash photolysis using Ru(bpy)3 as sensitizer and ascorbate as electron donor.
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Photoinduced electron injection in the medial FeS cluster of HupS was observed by EPR spectroscopy.