Six new steroidal glycosides were obtained from Cynanchum stauntonii.
1–5 were new 13,14:14,15-disecopregnane-type C21 steroidal glycosides.
6 was new 14, 15-secopregnane-type C21 steroidal glycosides.
Some compounds could obviously inhibit iNOS and COX-2 expression.