Uptake and metabolism of fluorescent steroids by mycobacterial cells

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• 作者：Yaroslav Faletrov^a ; ^b ; ^{yaroslav82@tut.by} ; Anna Brzostek^c ; ^{abrzostek@cbm.pan.pl} ; Renata Plocinska^c ; ^{rplocinska@cbm.pan.pl} ; Jarosław Dziadek^c ; ^{jdziadek@cbm.pan.pl} ; Elena Rudaya^a ; ^b ; ^{lusova@mail.ru} ; Irina Edimecheva^b ; ^{shadiro@open.by} ; Vladimir Shkumatov^a ; ^b ; ^{biopharm@bsu.by}
• 关键词：BPCh ; TopFluor® ; BODIPY-cholesterol ; 23-(1 ; 3 ; 5 ; 7-tetramethyl-4 ; 4-difluoro-4-bora-3a ; 4a-diaza-s-indacene)-24-norcholesterol ; 22NC ; 22-NBD-cholesterol ; 22-(N-(7-nitrobenz-2-oxa-1 ; 3-diazol-4-yl)amino)-23 ; 24-bisnorcholesterol ; 20NP ; 20-((7-nitrobenz-2-oxa-1 ; 3-diazol-4-yl)amino)-pregn-5-en-3β-ol ; 3NC ; 3-((7-nitrobenz-2-oxa-1 ; 3-diazol-4-yl)amino)-5α-cholestane
• 刊名：Steroids
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：117
• 期：Complete
• 页码：29-37
• 全文大小：2199 K
• 卷排序：117

文摘

Mycobacteria can convert BODIPY-cholesterol and similar NBD-labeled steroids. 4-En-3-one derivatives are major fluorescent products of the bioconversion. NBD fluorophore of the compounds undergoes degradation in the mycobacteria. The steroids cause staining of envelope lipids and intracellular lipid droplets. 3-NBD-cholestane don’t converted by M. tuberculosis H37Rv and M. smegmatis mc2 155.