Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200 kV

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• 作者：Xiaowu Li¹ ; ² ; Niyun Zhou³ ; Wenyuan Chen¹ ; Bin Zhu¹ ; Xurong Wang¹ ; Bin Xu² ; Jiawei Wang³ ; Hongrong Liu¹ ; ^{hrliu@hunnu.edu.cn} ; Lingpeng Cheng² ; ³ ; ^{lingpengcheng@mail.tsinghua.edu.cn}
• 关键词：cryo-EM ; cryo-electron microscopy ; DED ; direct electron detection ; CCD ; charge-coupled device ; EM ; electron microscopy ; TEM ; transmission electron microscope ; dsRNA ; double-stranded RNA ; RdRp ; RNA-dependent RNA polymerase ; DQE ; detective quantum efficiency ; PR ; phase residual ; CC ; correlation coefficient ; FSC ; Fourier shell correlation
• 刊名：Journal of Molecular Biology
• 出版年：2017
• 出版时间：6 January 2017
• 年：2017
• 卷：429
• 期：1
• 页码：79-87
• 全文大小：1518 K
• 卷排序：429

文摘

The cypovirus capsid structure was resolved at 3.3-Å resolution using a 200-kV TEM. The criterion for particle image selection was proposed. The structure of RdRp complex within the capsid was resolved at 3.9-Å resolution. The conformational change of RdRp suggests that the RdRp might also function as an RNA helicase.