The two Dictyostelium autophagy eight proteins, ATG8a and ATG8b, associate with the autophagosome in succession

详细信息    查看全文

• 作者：Jan Matthias ; Susanne Meß ; ling ; Ludwig Eichinger ; ^{ludwig.eichinger@uni-koeln.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Dictyostelium discoideum ; Autophagy ; ATG8/LC3 ; ATG9 ; Ubiquitin ; Live cell imaging
• 刊名：European Journal of Cell Biology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：95
• 期：1
• 页码：15-25
• 全文大小：2753 K

文摘

Autophagy is an ancient cellular pathway that is conserved from yeast to man. It contributes to many physiological and pathological processes and plays a major role in the degradation of proteins and/or organelles in response to starvation and stress. In the autophagic process cytosolic material is captured into double membrane-bound vesicles, the autophagosomes. After fusion with lysosomes, the cargo is degraded in the generated autolysosomes and then recycled for further use.

Autophagy 8 (ATG8, in mammals LC3), a well-established marker of autophagy, is covalently linked to phosphatidylethanolamine on the autophagic membrane during autophagosome formation. Bioinformatic analysis of the Dictyostelium genome revealed two atg8 genes which encode the ATG8a and ATG8b paralogs. They are with around 14 kDa similar in size, 54 % identical to one another and more closely related to the corresponding proteins in fungi and plants than in animals. For ATG8a we found a strong up-regulation throughout the 24 h developmental time course while ATG8b expression was highest in vegetative cells followed by a moderate reduction during early development. Confocal microscopy of fluorescently tagged ATG8a and ATG8b in vegetative AX2 wild-type and in ATG9⁻ cells showed that both proteins mainly co-localized on vesicular structures with a diameter above 500 nm while those smaller than 500 nm were predominantly positive for ATG8b. In ATG9⁻ cells we found a strong increase in the relative abundance of ATG8a-positive large vesicular structures and of total ATG8b-positive structures per cell indicating autophagic flux problems in this mutant. We also found that vesicular structures positive for ATG8a and/or ATG8b were also positive for ubiquitin. Live cell imaging of AX2 and ATG9⁻ cells co-expressing combinations of red and green tagged ATG8a, ATG8b or ATG9 revealed transient co localizations of these proteins. Our results suggest that ATG8b associates with nascent autophagosomes before ATG8a. We further find that the process of autophagosome formation in Dictyostelium is highly dynamic. We infer from our data that Dictyostelium ATG8a and ATG8b have distinct functions in autophagosome formation and that ATG8b is the functional orthologue of the mammalian LC3 subfamily and ATG8a of the GABARAP subfamily.