Effect of freezing rate for cryopreservation of Persian sturgeon (Acipenser persicus) spermatozoa

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• 作者：Mohammad Sadegh Aramli^a ; ^{msaramli@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Karim Golshahi^b ; Rajab Mohammad Nazari^c ; Ebrahim Sotoudeh^d ; Salim Aramli^e ; Ensieh Habibi^f
• 关键词：Sperm motility ; Fertility ; ATP ; Oxidative stress ; Cryopreservation ; Persian sturgeon
• 刊名：Theriogenology
• 出版年：2016
• 出版时间：1 March 2016
• 年：2016
• 卷：85
• 期：4
• 页码：734-739
• 全文大小：401 K

文摘

This study examined the effect of freezing rate (−10 °C, −15 °C, −20 °C, −30 °C, and −40 °C/min) on motility parameters, rates of fertilization and hatching, ATP content, and indices of oxidative stress including thiobarbituric acid reactive substances and carbonyl derivatives of proteins in Persian sturgeon (Acipenser persicus) sperm. After sampling, sperm was diluted in an extender composed of 23.4-mM sucrose, 0.25-mM KCl, and 30-mM Tris-HCl, pH 8.0, containing 10% methanol and subsequently frozen in a programmable freezer. For postthaw sperm that were frozen at a rate of −40 °C/min, sperm motile duration (134 ± 27.01 seconds), sperm motile percent (60 ± 4.1%), fertilizability (72 ± 8.36% for fertilization rate and 65 ± 7.58% for hatching rate), and ATP content (4.8 ± 0.57 nmol/10⁸ sperm) were significantly higher than for sperm frozen at any of the four slower rates (P < 0.05). Moreover, sperm cryopreserved using the fastest freezing rate had significantly lower levels of thiobarbituric acid reactive substances (0.5 ± 0.05 nmol/10⁸ sperm) and carbonyl derivatives of proteins (41.3 ± 4.9 nmol/10⁸ sperm) than sperm cryopreserved using all other freezing rates (P < 0.05). In addition, there is a significant difference (P < 0.05) between fresh sperm and the recovery of cryopreserved Persian sturgeon sperm using programmable freezing with −40 °C/min being the optimal freezing rate among those tested.