An efficient Horseradish peroxidase chemiluminescence biosensor with surface imprinting based on phenylboronic acid functionalized ionic liquid@magnetic graphene oxide

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• 作者：Huimin DuanAuthor Vitae ; Xiaojiao WangAuthor Vitae ; Yanhui WangAuthor Vitae ; Jianbo LiAuthor Vitae ; Yuanling SunAuthor Vitae ; Chuannan Luo ; ^{chm_luocn@ujn.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Phenylboronic acid boronate affinity ; Ionic liquid ; Horseradish peroxidase ; Surface molecular imprinting ; Chemiluminescence biosensor ; Magnetic graphene oxide
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：29 November 2016
• 年：2016
• 卷：236
• 期：Complete
• 页码：44-51
• 全文大小：1941 K

文摘

Mainly, ionic liquid (IL) was used as functional bridging agent and surface modifier to obtain phenylboronic acid modified-IL functionalized GO@Fe₃O₄ nanoparticles (GO@Fe₃O₄@IL/PBA) with reversible covalent binding properties with cis-diol containing compounds particularly. Then GO@Fe₃O₄@IL/PBA with the properties of high surface area and easy separation was designed as supporting material to prepare Horseradish Peroxidase (HRP) SMIP which then was characterized by SEM, XRD and FTIR. The adsorption capacity of GO@Fe₃O₄@IL/PBA-SMIP to HRP was researched to be 8.8 g/g, and the adsorption model and adsorption kinetics of GO@Fe₃O₄@IL/PBA-SMIP to HRP followed Langmuir adsorption isotherm and pseudo second-order model while the washing process was superior with acetic acid than NaOH solutions. Possessing the merit of improving selectivity, GO@Fe₃O₄@IL/PBA-SMIP was introduced into CL biosensor which could linearly respond to HRP in the range of 1.0 × 10⁻⁴–8.0 × 10⁻³ mg/mL with a detection limit 2.9 × 10⁻⁵ mg/mL. Finally, the proposed GO@Fe₃O₄@IL/PBA-SMIP-CL biosensor was used to detect the HRP in samples with satisfied results and the recoveries were ranged from 87% to 107%.