Detection of human leptin in serum using chemiluminescence immunosensor: Signal amplification by hemin/G-quadruplex DNAzymes and protein carriers by Fe3O4/polydopamine/Au nanocomposites
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- 作者：Yuezhen HeAuthor Vitae ; Jian SunAuthor Vitae ; Xiaoxun WangAuthor Vitae ; Lun Wang ; wanglun@mail.ahnu.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Human leptin ; Biosensor ; Magnetic nanocomposite ; Fe3O4/polydopamine/Au ; G-quadruplex ; DNAzymes
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2015
- 出版时间：31 December 2015
- 年：2015
- 卷：221
- 期：Complete
- 页码：792-798
- 全文大小：1755 K
文摘
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The biosensor based on superparamagnetic Fe3O4@Au and hemin/G-quadruplex DNAzymes.
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Fe3O4@Au were prepared by in situ method with polydopamine as a substrate adhesion.
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DNAzymes were effective on catalyzing the chemiluminescence reaction of luminol–H2O2.
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Sensitive detection of leptin was achieved by the purification/amplification protocol.
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The sensing system held great potential for detecting other biomolecules of interest.
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