The effects of designed angiopoietin-1 variant on lipid droplet diameter, vascular endothelial cell density and metabolic parameters in diabetic db/db mice

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• 作者：Yu Jin Jung^a ; Hyun Ju Choi^a ; Jung Eun Lee^a ; Ae Sin Lee^a ; Kyung Pyo Kang^a ; ^c ; Sik Lee^a ; ^c ; Sung Kwang Park^a ; ^c ; Tae Sun Park^a ; ^c ; Heung Yong Jin^a ; ^c ; Sang Young Lee^b ; ^c ; Duk Hoon Kim^d ; Won Kim^a ; ^c ; ^{kwon@jbnu.ac.kr}
• 关键词：Angiopoietin-1 ; Fat droplet ; Endothelial cells ; Insulin resistance
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 出版时间：13 April, 2012
• 年：2012
• 卷：420
• 期：3
• 页码：498-504
• 全文大小：693 K

文摘

Metabolic syndrome consists of metabolic abnormality with central obesity, hypertriglyceridemia, insulin resistance and hypertension. Adipose tissue has been known as a primary site of insulin resistance and its adipocyte size may be correlated with the degree of insulin resistance. A designed angiopoietin-1, COMP-Angiopoietin-1 (COMP-Ang1), mitigated high-fat diet-induced insulin resistance in skeletal muscle. In this study, we examined effects of COMP-Ang1 on adipocyte droplet size, vascular endothelial cell density in adipose tissue and metabolic parameters in db/db mice by administering COMP-Ang1 or LacZ (as a control) adenovirus. Administration of COMP-Ang1 decreased fat droplet diameter in epididymal and abdominal visceral adipocyte and visceral fat content in db/db mice. The density of vascular endothelial cell in adipose tissue was increased in db/db mice after treatment with COMP-Ang1. Serum resistin and tumor necrosis factor-¦Á level was lower after treatment with COMP-Ang1 in db/db mice. COMP-Ang1 caused a restoration of fasting glycemic control in db/db mice and decreased serum insulin level and insulin resistance measured by HOMA index. These findings indicate that COMP-Ang1 regulates adipocyte fat droplet diameter, vascular endothelial cell density and metabolic parameters in db/db mice.