Copper-zinc-superoxide dismutase (CuZnSOD), an antioxidant gene from seahorse (Hippocampus abdominalis); molecular cloning, sequence characterization, antioxidant activity and potential peroxidation function of its recombinant protein
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- 作者：N.C.N. Pereraa ; b ; 1 ; G.I. Godahewaa ; b ; 1 ; Jehee Leea ; b ; jehee@jejunu.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Antioxidant ; CuZnSOD ; Flow cytometry ; MTT ; Peroxidation
- 刊名：Fish & Shellfish Immunology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：57
- 期：Complete
- 页码：386-399
- 全文大小：3671 K
文摘
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CuZnSOD was identified from seahorse (HaCuZnSOD).
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Optimum conditions for the antioxidant action may be pH 9 and 25 °C in seahorse.
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An MTT assay and flow cytometry confirmed that rHaCuZnSOD showed peroxidase activity in the presence of HCO3−.
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Transcriptional level of HaCuZnSOD was modulated by pathological stress.