In situ affinity purification of his-tagged protein A from Bacillus megaterium cultivation using recyclable superparamagnetic iron oxide nanoparticles

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• 作者：Johannes Gä ; dke^a ; ^b ; ^c ; ¹ ; Lennart Kleinfeldt^b ; ^d ; ¹ ; Chris Schubert^a ; ^b ; ^c ; ¹ ; Manfred Rohde^e ; Rebekka Biedendieck^c ; ^f ; Georg Garnweitner^b ; ^d ; ¹ ; Rainer Krull^a ; ^b ; ^c ; ¹ ; ^{r.krull@tu-braunschweig.de}
• 关键词：CFU ; colony forming unit ; DLS ; dynamic light scattering ; ELISA ; enzyme linked immunosorbent assay ; GNTA-SPION ; functionalized SPION analyzed in this article ; OD ; optical density ; SPION ; superparamagnetic iron oxide nanoparticles ; TGA ; thermogravimetric analysis
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：242
• 期：Complete
• 页码：55-63
• 全文大小：1001 K
• 卷排序：242

文摘

Functionalized SPION (GNTA-SPION) as sophisticated agent for product separation. Handheld magnets for fast SPION in situ removal from biotechnological process. One-step purification reaching high product purity using very low GNTA-SPION concentration. Successful GNTA-SPION regeneration and use in five consecutive cycles. No negative influence on bacterial growth and production behavior.