Production of cellulase by a newly isolated strain of Aspergillus sydowii and its optimization under submerged fermentation

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• 作者：Ketna Matkar^a ; ¹ ; Digantkumar Chapla^d ; Jyoti Divecha^b ; Anand Nighojkar^c ; Datta Madamwar^d ; ^{datta_madamwar@yahoo.com}
• 关键词：Aspergillus sydowii ; Cellulase ; Submerged fermentation ; Box&ndash ; Behnken design ; Response surface methodology
• 刊名：International Biodeterioration and Biodegradation
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：78
• 期：Complete
• 页码：24-33
• 全文大小：1034 K

文摘

The present work deals with the production of cellulase under submerged fermentation. A newly isolated strain was isolated from Indore, India and was found to be a potential cellulase producer. The isolate was identified as Aspergillus sydowii on basis of 18S ribosomal DNA sequencing. The medium for?cellulase production was optimized using response surface methodology and the predicted and verification value showed a difference of only 8.3 % validating the fitness of model. The filter paper activity?(1.33?IU/ml), endoglucanase (1.32?IU/ml), exoglucanase (3.99?IU/ml) and ¦Â-glucosidase (cellobiase - 9.24?IU/ml) were maximum on 6th day under SmF using 10 % (v/v) inoculum with 0.1 % Tween-20 at 40?¡ãC, pH 5.5 and 120?rpm. The ratio of endoglucanase, exoglucanase and ¦Â-glucosidase produced under optimized conditions was 9:27:64. The FPA:Cb ratio of 1:7.1 indicated a high potential of enzyme for saccharification. Amongst various carbon sources used lactose was found to have an induction effect on cellulase production.