Synchrotron-induced X-ray microfluorescence on single cells
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- 作者：Bohic ; Sylvain ; Simionovici ; Alexandre ; Ortega ; Richard ; Heymann ; Dominique ; Schroer ; Christian ; et. al.
- 关键词：78.70.E ; 41.60.Ap ; 07.85.Qe ; Microprobe ; X-ray fluorescence ; Synchrotron ; Cancer cells ; Doxorubicin
- 刊名：Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
- 出版年：2001
- 出版时间：July, 2001
- 年：2001
- 卷：181
- 期：1-4
- 页码：728-733
- 全文大小：430 K
文摘
Recent improvements in synchrotron X-ray sources (third generation) and in X-ray focusing elements have been realized. This result in delivering highly collimated quasi-monochromatic X-ray beams with tunable energy and beam focused down to sub-micrometer diameter. Preliminary results in the μ-SXRF of single cells were obtained in the hard X-ray range. “Pink”-beam and compound refractive lenses were used resulting in a 1×10μm2 (vertical×horizontal) beam size with a flux 
5×1010photon/s/μm2. The experiment confirmed that high energy, high intensity X-rays were well suited for microanalysis of sensitive biological specimens such as freeze-dried cells. Results show that the synchrotron microprobe set-up at ESRF allows high sensitivity in trace element measurements for cells treated with pharmacological doses of an iodine-labeled anticancer drug.
5×1010photon/s/μm2. The experiment confirmed that high energy, high intensity X-rays were well suited for microanalysis of sensitive biological specimens such as freeze-dried cells. Results show that the synchrotron microprobe set-up at ESRF allows high sensitivity in trace element measurements for cells treated with pharmacological doses of an iodine-labeled anticancer drug.