The effect of inflammation on sympathetic nerve mediated contractions in rat isolated caudal artery

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• 作者：Jocelyn Fotso Soh^a ; Hilary R. Strong^a ; Noriko Daneshtalab^a ; ^b ; ^{norikod@mun.ca} ; Reza Tabrizchi^a ; ^{rtabrizc@mun.ca}
• 关键词：Inflammation ; Complete Freund's adjuvant ; Caudal artery ; Alpha1 and alpha2B adrenergic receptors ; Sympathetic nerves ; Neurogenic contractions
• 刊名：European Journal of Pharmacology
• 出版年：2016
• 出版时间：5 December 2016
• 年：2016
• 卷：792
• 期：Complete
• 页码：54-62
• 全文大小：1158 K
• 卷排序：792

文摘

Chronic inflammatory process(es) contributes to changes in vascular function in a variety of diseases. Sympathetic nerve-mediated responses in blood vessels play a pivotal role in regular physiological functions. We tested the hypothesis that sympathetic neuro-effector function will be altered as consequence of inflammatory state. Sympathetic nerve-mediated contractions and alpha adrenergic receptor expressions were evaluated in isolated caudal arteries of rats treated with saline and Complete Freund's adjuvant (CFA). While CFA-treated animals had significantly higher plasma levels of tumor necrosis factor-alpha compared to saline, blood pressure remained unchanged. Immunofluorescence revealed increased expression of ionized calcium adapter binding molecule-1 in the adventitia of blood vessels from CFA-treated animals compared to saline. In isolated arteries, electrical field stimulations between 1.25 and 40 Hz resulted in frequency-dependent contractions that wasabolished by tetrodotoxin. Neurogenic contractions from CFA groups were significantly greater than saline. While the presence of alpha1-adrenoceptor antagonist (prazosin) significantly inhibited contractions at lower frequencies of stimulation (1.25–5 Hz) in isolated arteries of CFA-treated rats compared to controls, alpha2-adrenoceptor antagonist (rauwolscine) had modest effects. Inhibition of neuronal reuptake by cocaine comparably enhanced field-stimulated responses in vessels of experimental and control animals. Immunofluorescence revealed a difference in expression of alpha1- and alpha2-adrenoceptors in the endothelium of blood vessels of CFA compared to saline controls. Collectively, our observations lend support to enhanced neurogenic contractions in blood vessels of inflamed animals possibly attributing to alterations in responsiveness and/or distribution of post-junctional alpha1-adrenoceptors.